CALCA polyclonal antibody

Calcitonin is a 32 amino acid polypeptide hormone that preserves skeletal integrity and reduces blood calcium levels by decreasing osteoclast activity in bones, calcium and phosphate reabsorption by kidney tubules and calcium absorption by the intestines. The secretion of Calcitonin from the thyroid is regulated in part by estrogen, which increases Calcitonin mRNA levels. The Calcitonin gene, CALCA, undergoes tissue-specific RNA alternative splicing, resulting in the production of different mRNA transcripts. One transcript encodes procalcitonin as well as both calcium-lowering processed active polypeptides, Calcitonin and katacalcin. An alternative transcript of CALCA encodes the precursor for the neuropeptide referred to as Calcitonin gene-related peptide 1, also designated CGRP1 or å-CGRP. CGRP is a widely distributed vasodilatory peptide. Calcitonin and katacalcin are produced primarily in the thyroid, while CGRP is produced in neuronal cells. A second CGRP related gene, CALCB, thought to be derived from an gene duplication event, has been identified in mouse, rat and human. Unlike CALCA, CALCB is not subject to alternative splicing and encodes a single transcript designated CGRP2 or β-CGRP. Mature CGRP1 and CGRP2 share significant sequence identity at the protein level differing by only 1-3 amino acid residues, depending on the species.

9007-83-4

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Calcitonin gene-related peptide 1; Alpha-type CGRP; Calcitonin gene-related peptide I; CGRP-I; CALCA; CALC1

P06881/P01258

Human, Rat

A synthetic peptide corresponding to residues in Human CALCA.

Unconjugated

WB

WB: 1:500~1:1000

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Unmodification

~ 13 kDa

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.

CALCA polyclonal antibody detects endogenous levels of CALCA protein.

Western blot (WB) analysis of CALCA polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate Lane2:H9C2 whole cell lysate