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Sarcoglycan-α polyclonal antibody

CAT: 0384-BS5920-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS5920-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The sarcoglycan transmembrane proteins are members of the dystrophin complex. Sarcoglycans cluster together to form a complex, which is localized in the cell membrane of skeletal, cardiac, and smooth muscle fibers. Four sarcoglycan subunit proteins, designated α-, β-, γ- and δ-sarcoglycan, form a complex on the skeletal muscle cell surface membrane. A genetic defect in any one of these proteins causes the loss or marked decrease of the whole sarcoglycan complex, which is observed in the autosomal recessive muscular dystrophy, sarcoglycanopathy. In smooth muscle, β- and δ-sarcoglycans are associated with ε-sarcoglycan, a glycoprotein homologous to α-sarcoglycan. Additionally, a complete deficiency in δ-sarcoglycan is the cause of the Syrian hamster BIO.14 cardiomyopathy.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Alpha-sarcoglycan; Sarcoglycan α; α-Sarcoglycan; α-SG; SG-α; SGα; Alpha-SG; 50 kDa dystrophin-associated glycoprotein; 50DAG; Adhalin; Dystroglycan-2; Dystroglycan 2; Dystroglycan2; SGCA; ADL; DAG2
Swiss Prot
Q16586
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 209-254 of Human Sarcoglycan-α.
Conjugation
Unconjugated
Applications
WB ICC IP
Dilution
WB: 1:500~1:1000 ICC: 1:50~1:200 IP: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 42 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Sarcoglycan-α polyclonal antibody detects endogenous levels of Sarcoglycan-α protein.
Applications Notes
Western blot (WB) analysis of Sarcoglycan-α pAb at 1:500 dilution Lane1:AML-12 whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:EC9706 whole cell lysate (40ug) Lane5:H1792 whole cell lysate (40ug)
Host or Source
Rabbit

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