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PCB polyclonal antibody

CAT: 0384-BS5841-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS5841-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Pyruvate carboxylase (PCB) is a biotinylated mitchondrial enzyme that catalyzes the synthesis of oxaloacetate from pyruvate in a tissue specific manner. In addition to covalently binding the biotin cofactor, PCB contains consensus sequences for the attachment of ATP and the substrate pyruvate. The PCB gene is located on the long arm of chromosome 11. Mutations in PCB metabolism (pyruvate carboxylase deficiency) are known to cause lactic acidosis, hypoglycemia and mental retardation.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Pyruvate carboxylase, mitochondrial; Pyruvic carboxylase; PCB; PC
Swiss Prot
P11498
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 400-445 of Human PCB.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 130 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PCB polyclonal antibody detects endogenous levels of PCB protein.
Applications Notes
Western blot (WB) analysis of PCB polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:NIH-T3 cell lysate Lane3:H9C2 cell lyate
Host or Source
Rabbit

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