Catenin-β (phospho-T41/S45) polyclonal antibody

• Background:

  β-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Catenin β; Cateninβ; Catenin beta-1; Beta-catenin; CTNNB1; CTNNB; ORF Names: OK/SW-cl.35; PRO2286

• Swiss Prot:

  P35222

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A phospho specific peptide corresponding to residues surrounding threonine 41 and 45 of Human Catenin-β.

• Conjugation:

  Unconjugated

• Related Pathways:

  Hypoxia Signaling, Wntβ catenin signaling, Contribution of ECM and Cytoskeletal Factors to EMT, Contribution of Soluble Factors to EMT, hippo Signaling, Adherens Junction signaling

• Applications:

  IHC

• Dilution:

  IHC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylation

• Molecular Weight:

  ~ 92 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  P-beta-catenin (T41/S45) polyclonal antibody detects endogenous levels of beta-catenin protein only when phosphorylated at Thr41/S45.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4