CD19 (phospho-Y531) polyclonal antibody

• Background:

  CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is selectively expressed on the cell surface of B-lymphocytes, where it activates intracellular signaling cascades involving both Ras and phosphatidylinositol 3-kinase pathways. Activation of CD19 results in cross-linking of the membrane protein immunoglobulin chains and the subsequent association with Src family protein tyrosine kinases (PTK) . Expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. CD19 forms functional complexes with B-lymphocyte surface proteins, including integrin β1, CD21 and CD81, which are involved in regulating B-cell development.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12

• Swiss Prot:

  P15391

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic phosphopeptide derived from human CD19 around the phosphorylation site of Tyrosine 531.

• Conjugation:

  Unconjugated

• Related Pathways:

  B cell receptor signaling

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylation

• Molecular Weight:

  ~ 61, 95 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  P-CD19 (Y531) polyclonal antibody detects endogenous levels of CD19 protein only when phosphorylated at Tyr531.

• Applications Notes:

  Western blot (WB) analysis of p-CD19 (Y531) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with EGF (0.1ng/ML,30mins) Lane2:Raw264.7 cell lysate treated with EGF (0.1ng/ML,30mins) Lane3:H9C2 cell lysate treated with EGF (0.1ng/ML,30mins)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4