PKC α (phospho-T505) polyclonal antibody

• Background:

  Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ) . Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Protein kinase C alpha type; PKC-A; PKC-alpha; PRKCA; PKCA; PRKACA

• Swiss Prot:

  P17252

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic phosphopeptide derived from human PKC α around the phosphorylation site of Threonine 505.

• Conjugation:

  Unconjugated

• Related Pathways:

  Phospholipase Signaling, Protein Kinase C Signaling, Inhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk, Mitochondrial Control of Apoptosis

• Applications:

  WB IHC

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylation

• Molecular Weight:

  ~ 80 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  P-PKC α (T505) polyclonal antibody detects endogenous levels of PKC α protein only when phosphorylated at Thr505.

• Applications Notes:

  Western blot (WB) analysis of p-PKC α (T505) pAb at 1:500 dilution Lane1:Hela whole cell lysate (10ug) Lane2:The Kidney tissue lysate of Mouse (40ug) Lane3:The Heart tissue lysate of Rat (20ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4