GSK3α (phospho-S21) polyclonal antibody

• Background:

  Glycogen synthase kinase-3 α and β (GSK-3α, β) are serine/threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3β activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFα in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3α and GSK-3β induces phosphorylation of Ser 21 and Ser 9, respectively through a phosphatidylinositol 3-kinase (PI 3-kinase) -protein kinase B (PKB) dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3α or Ser 9 of GSK-3β and inactivates both forms

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A

• Swiss Prot:

  P49840

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic phosphopeptide derived from human GSK3α around the phosphorylation site of Serine 21.

• Conjugation:

  Unconjugated

• Related Pathways:

  Translational Contral elF4 and p70 S6 Kinase, Regulation of Microtubule Dynamics

• Applications:

  WB IHC IP

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylation

• Molecular Weight:

  ~ 51 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  P-GSK3α (S21) polyclonal antibody detects endogenous levels of GSK3α protein only when phosphorylated at Ser21 .

• Applications Notes:

  Western blot (WB) analysis of p-GSK3α (S21) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with UV Lane2:Mouse liver tissue lysate Lane3:Rat liver tissue lysate Lane4:PC12 cell lysate treated with H2O2

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4