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PKC β (N655) polyclonal antibody

CAT: 0384-BS3708-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS3708-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ) . Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+. On the other hand, nPKC δ and ε, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Protein kinase C beta type; PKC-B; PKC-beta; PKCB; PRKCB1; PRKCB
Swiss Prot
P05771
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 621-670 of Human PKC β.
Conjugation
Unconjugated
Related Pathways
Phospholipase Signaling, Inhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk, Mitochondrial Control of Apoptosis
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 82 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PKC β (N655) polyclonal antibody detects endogenous levels of PKC β protein.
Applications Notes
Western blot (WB) analysis of PKC β (N655) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate
Host or Source
Rabbit

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