AGER / RAGE (E182) polyclonal antibody

• Background:

  Advanced glycosylation end products of proteins (AGEs) are nonenzymatically glycosylated proteins that are associated with a variety of conditions, including diabetes and other vascular disorders, as well as amyloidosis. These proteins regulate cellular functions via specific cell surface acceptor molecules, such as RAGE (receptor for advanced glycosylation end products) . RAGE is a type 1 membrane protein that is found on the surface of endothelial cells, mononuclear phagocytes and vascular smooth muscle cells. Binding of AGEs to RAGE results in the induction of cellular oxidant stress and activation of the transcription factor NFkB. Evidence suggests that the induction of oxidant stress results in the activation of an intracellular cascade involving p21 ras and MAP kinase, which leads to activation of transcription.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Receptor for advanced glycosylation end products; Advanced glycosylation end product-specific receptor; AGER; RAGE

• Swiss Prot:

  Q15109

• Reactivity:

  Human

• Immunogen:

  Synthetic peptide, corresponding to amino acids 150-205 of Human AGER / RAGE.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~43 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  AGER (E182) polyclonal antibody detects endogenous levels of AGER protein.

• Applications Notes:

  Western blot (WB) analysis of RAGE (E182) polyclonal antibody in extracts from HEK293A cells.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4