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NOS3 (I611) polyclonal antibody

CAT: 0384-BS3259-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS3259-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS) ; inducible NOS (iNOS) or macrophage NOS (mNOS) ; and endothelial NOS (eNOS) .eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII; NOS3
Swiss Prot
P29474
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 580-630 of Human NOS3.
Conjugation
Unconjugated
Related Pathways
Hypoxia Signaling, Tumor Angiogenesis
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 140 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
NOS3 (I611) polyclonal antibody detects endogenous levels of NOS3 protein.
Applications Notes
Western blot (WB) analysis of NOS3 (I611) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate
Host or Source
Rabbit

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