ERAB (E135) polyclonal antibody

• Background :

  β-Amyloid is a neurotoxic peptide that is associated with the pathogenesis of Alzheimer’s disease. β-Amyloid aggregates induce cell death of neurons through the disruption of cell membranes and the generation of reactive oxygen intermediates.These neurotoxic effects are also attributed to the interaction of β-Amyloid with intracellular proteins, specifically ERAB, the endoplasmic reticulum-associated β-Amyloid-binding protein. ERAB is characterized as a NAD+-dependent dehydrogenase that is constitutively expressed in tissues and overexpressed in neurons affected in Alzheimer’s disease. Cells overexpressing ERAB in vitro have been shown to be more sensitive to β-Amyloid-induced stress, and blocking the activity of ERAB has been shown to inhibit this cell death, indicating that β-Amyloid induced cell death is mediated by ERAB.

• CAS Number :

  9007-83-4

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  3-hydroxyacyl-CoA dehydrogenase type-2; 17-beta-hydroxysteroid dehydrogenase 10; 17-beta-HSD 10; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase; 3-hydroxyacyl-CoA dehydrogenase type II; Endoplasmic reticulum-associated amyloid beta-peptide-binding protein; Mitochondrial ribonuclease P protein 2; Mitochondrial RNase P protein 2; Short-chain type dehydrogenase/reductase XH98G2; Type II HADH; HSD17B10; ERAB; HADH2; MRPP2; SCHAD; XH98G2

• Swiss Prot :

  Q99714

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthetic peptide, corresponding to amino acids 102-150 of Human ERAB.

• Conjugation :

  Unconjugated

• Applications :

  WB IHC

• Dilution :

  WB: 1:500~1:1000 IHC: 1:50~1:200

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 27 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  ERAB (E135) polyclonal antibody detects endogenous levels of ERAB protein.

• Applications Notes :

  Western blot (WB) analysis of ERAB (E135) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate

• Host or Source :

  Rabbit