CrkL (P201) polyclonal antibody

CAT: 0384-BS1706-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS1706-01Size:50 µL
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Background
Crk, is a transformation-specific protein that induces elevation of cellular phosphotyrosine levels, but lacks tyrosine kinase activity itself. A second protein, Nck, consists solely of three SH3 domains and one SH2 domain, while GRB2 contains an SH2 domain flanked on both sides by SH3 domains. A member of this protein class, Crk-L, is encoded by a gene located on chromosome 22, band 11, centromeric of the chronic myelogenous leukemia breakpoint region. Crk-L encodes a 303 amino acid protein with one SH2 and two SH3 domains, and a predicted molecular weight of 36 kDa.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Crk L; Crk like protein; Crk-like protein; Crkl; Crkol; Oncogene CrkL
Swiss Prot
P46109
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 180-230 of Human CrkL.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 39 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
CrkL (P201) polyclonal antibody detects endogenous levels of CrkL protein.
Applications Notes
Western blot (WB) analysis of CrkL (P201) pAb at 1:500 dilution Lane1:K562 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug)
Host or Source
Rabbit

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