TGFβ RII (E246) polyclonal antibody

• Background :

  A total of three members of the TGFβ family, TGFβ1, TGFβ2 and TGFβ3, have been identified in mammals. Each is synthesized as a latent precursor that is subsequently cleaved forming the 112 amino acid growth factor which becomes active upon dimerization. TGFβs mediate their activity by high affinity binding to the type II receptor (TGFβ RII) transmembrane protein with a cytoplasmic serine-threonine kinase domain. For signaling growth inhibition and early gene responses the type II receptor requires both its kinase activity and association with a TGFβ-binding protein, designated the type I receptor. Two independent groups have recently described the cloning and sequence analysis of genes encoding TGFβ type I receptor proteins designated ALK-5 (TβR-1) and TSR-1, respectively.

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  TGF-beta receptor type-2; TGFR-2; TGFR2; TGF-beta type II receptor; Transforming growth factor-beta receptor type II; TGF-beta receptor type II; TbetaR-II; TGFBR2

• Swiss Prot :

  P37173

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthetic peptide, corresponding to amino acids 220-300 of Human TGFβ RII.

• Conjugation :

  Unconjugated

• Related Pathways :

  Contribution of Soluble Factors to EMT

• Applications :

  WB IHC

• Dilution :

  WB: 1:500~1:1000 IHC: 1:50~1:200

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 65 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  TGFβ RII (E246) polyclonal antibody detects endogenous levels of TGFβ RII protein.

• Applications Notes :

  Western blot (WB) analysis of TGFβ RII (E240) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:H9C2 whole cell lysate

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4