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Raf-1 (S291) polyclonal antibody

CAT: 0384-BS1623-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS1623-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1; RAF1; RAF
Swiss Prot
P04049
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 261-310 of Human Raf-1.
Conjugation
Unconjugated
Related Pathways
Inhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk, hippo Signaling, B cell receptor signaling, MAPK Erk in Growth and Differentiation Pathway
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 73 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Raf-1 (S291) polyclonal antibody detects endogenous levels of Raf-1 protein.
Applications Notes
Western blot (WB) analysis of Raf-1 (S291) polyclonal antibody at 1:500 dilution Lane1:MEF whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug)
Host or Source
Rabbit

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