Gab1 (E655) polyclonal antibody

• Background :

  Gab 1 (GRB2-associated binder 1), one such member of the IRS family, plays an important role in cellular growth response, transformation and apoptosis. Gab 1 is a multi-substrate docking protein that functions downstream in the signaling pathways of different receptor kinases, including EGFR. Gab1 is tyrosine phosphorylated normally in response to insulin and consequently enhances phosphatidylinositol 3- kinase (PI3K) binding. In response to osmotic shock, tyrosine-phosphorylated Gab 1 (p-Gab 1) also binds and activates phosphatidylinositol 3-kinase, suggesting that Gab 1 is the major site for PI3K recruitment following osmotic shock stimulation.

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  GRB2-associated-binding protein 1; GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1; GAB1

• Swiss Prot :

  Q13480

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthetic peptide, corresponding to amino acids 622-672 of Human Gab1.

• Conjugation :

  Unconjugated

• Applications :

  WB IHC IF

• Dilution :

  WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 76, 110 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  Gab1 (E655) polyclonal antibody detects endogenous levels of Gab1 protein.

• Applications Notes :

  Western blot (WB) analysis of Gab1 (E655) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:H9C2 whole cell lysate

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4