Cdc25B (R317) polyclonal antibody

• Background:

  The activity of cyclin dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and Cdc25 phosphatases. Three Cdc25 genes are present in human cells: Cdc25A, Cdc25B and Cdc25C. Cdc25A and Cdc25B are expressed throughout the cell cycle, with peak expression in G1 for Cdc25A and in both G1 S phase and G2 for Cdc25B. Cdc25C is mainly expressed in G2 phase.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B; CDC25B; CDC25HU2

• Swiss Prot:

  P30305

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 280-340 of Human Cdc25B.

• Conjugation:

  Unconjugated

• Related Pathways:

  G Protein-coupled Receptors Signaling to MAPK Erk, Cell Cycle G2 M DNA Damage Signaling Pathway

• Applications:

  IHC IF

• Dilution:

  IHC: 1:50~1:200 IF: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 65 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CDC25B (R317) polyclonal antibody detects endogenous levels of CDC25B protein.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4