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ATF4 (R239) polyclonal antibody

CAT: 0384-BS1026-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS1026-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB2) . The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain (referenced from Entrez gene) .
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Cyclic AMP-dependent transcription factor ATF-4; cAMP-dependent transcription factor ATF-4; Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67; TaxREB67; ATF4; CREB2; TXREB
Swiss Prot
P18848
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 200-250 of Human ATF4.
Conjugation
Unconjugated
Related Pathways
Insulin &Glucose Signaling
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 49 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ATF4 (R239) polyclonal antibody detects endogenous levels of ATF4 protein.
Applications Notes
Western blot (WB) analysis of ATF4 pAb at 1:5000 dilution Lane1:EC9706 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:A375 whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug)
Host or Source
Rabbit

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