LC3 (phospho-Thr50) polyclonal antibody

• Background:

  Two forms of LC3β , the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3β , followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3’ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3β may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Microtubule-associated protein 1 light chain 3 beta; MAP1LC3B; MAP1ALC3; LC3-I; LC3-II; LC3I; LC3II; MAP1LC3A

• Swiss Prot:

  Q9GZQ8/Q9H492/Q9BXW4

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic phosphopeptide derived from human LC3 around the phosphorylation site of Threonine 50.

• Conjugation:

  Unconjugated

• Related Pathways:

  Phosphoinosotode Signaling, Autophagy Signaling

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylation

• Molecular Weight:

  ~ 15 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  LC3 (phospho-Thr50) polyclonal antibody detects endogenous levels of LC3A and LC3B protein only when phosphorylated at Thr50. This antibody also recognizes LC3C when phosphorylated at the corresponding residues.

• Applications Notes:

  Western blot (WB) analysis of LC3 (phospho-Thr50) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:L02 treated with PBS (1xPBS, PH7.4) for 1 hour whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:HEK293T treated with Etoposide (20ng/ml) for 30 minutes whole cell lysate (40ug) Lane5:HEK293T treated with Etoposide (20ng/ml) for 6 hours whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4