Products for Research Use Only
RegisterLogin

EIF5A2 (Y127) polyclonal antibody

CAT: 0384-BS9011-01Size: 50 µLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0384-BS9011-01Size:50 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
In mammalian cells, translation is controlled at the level of polypeptide chain initiation by eukaryotic initiation factors. The translation initiation factor 5 (eIF5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal subunit, a function necessary for the subsequent joining of the 40S and 60S subunits to form the 80S initiation complex. eIF4E specifically binds to the mRNA cap to promote unwinding and exposure of the AUG-initiation codon. Overexpression of eIF4E can lead to cell transformation and tumorigenesis. An additional initiation factor, eIF2, is present as a heterotrimer composed of eIF2α, eIF2β and eIF2γ subunits. This heterotrimer forms a complex with GTP and tRNA which then binds to the 40S ribosomal subunit. After the formation of the 80S initiation complex, eIF2 is hydrolyzed and eIF2-GDP is released from the complex. eIF2-GDP is subsequently converted to eIF2-GTP, a reaction catalyzed by eIF2B, and is then available to catalyze another round of initiation.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Eukaryotic translation initiation factor 5A-2; eIF-5A-2; eIF-5A2; Eukaryotic initiation factor 5A isoform 2; EIF5A2
Swiss Prot
Q9GZV4
Reactivity
Human, Rat, Mouse
Immunogen
Synthetic peptide, corresponding to amino acids 96-140 of Human eIF5A2.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 17 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
EIF5A2 (Y127) polyclonal antibody detects endogenous levels of IeIF5A2 protein.
Applications Notes
Western blot (WB) analysis of eIF5A2 (Y127) pAb at 1:500 dilution Lane1:AML-12 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:SGC7901 whole cell lysate (40ug) Lane5:HepG2 whole cell lysate (40ug) Lane6:A549 whole cell lysate (40ug) Lane7:A2780 whole cell lysate (40ug)
Host or Source
Rabbit

Related Products

CatalogName
BS71958-01EIF5A2 polyclonal antibody
PPL01494-3aPPLK/GFP+Puro-EIF5A2 shRNA-1
PPL01494-3dPPLK/GFP+Puro-EIF5A2 shRNA-4
PPL01494-3bPPLK/GFP+Puro-EIF5A2 shRNA-2
PPL01494-3cPPLK/GFP+Puro-EIF5A2 shRNA-3
BS3022-01CD9 (Y129) polyclonal antibody
BS70573-01EIF4A2 polyclonal antibody
BS7351-01EIF5A polyclonal antibody
BS60424-01EIF5A polyclonal antibody
BS90456eIF5A polyclonal antibody

Alternative Products

CatalogName
GTR18040628-01EIF5A2 (Y127) polyclonal antibody
A05688Y127Anti-eIF5A2 (Y127) Antibody
APG00094GPolyclonal Goat Anti-EAT2 phospho (Y127) Antibody
JOT-AP02902-01eIF5A2 Polyclonal Antibody
NBP1-85943Rabbit Polyclonal eIF5A2 Antibody
abx432633EAT2 phospho (Y127) Antibody
R34919-100UGPhospho-EAT2 Antibody (Y127)

Popular Products