ERK3 (phospho-S189) polyclonal antibody

• Background:

  Mitogen-activated protein kinase (MAPK) signaling pathways involve closely related MAP kinases, including extracellular-signal-related kinase 3 (ERK 3, also designated PRKM6 and p97MAPK) . Serum, growth factors and phorbol esters can initiate ERK 3 signaling pathways. Despite lacking a definitive nuclear localization sequence, ERK 3 constitutively localizes to the nucleus upon activation. p38 pathway activation-dependent upregulation of ERK 3 is independent of the status of p53, Bcl2 and caspase 3 during cell stress and damage induced by proteasome inhibition, suggesting ERK 3 in part mediates intracellular defense or cell rescue. The human ERK3 gene maps to chromosome 15q21 and encodes a 721 amino acid protein.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Mitogen-activated protein kinase 6; MAP kinase 6; MAPK 6; Extracellular signal-regulated kinase 3; ERK-3; MAP kinase isoform p97; p97-MAPK; MAPK6; ERK3; PRKM6

• Swiss Prot:

  Q16659

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic phosphopeptide derived from human ERK3 around the phosphorylation site of Serine 189.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:2000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylation

• Molecular Weight:

  ~ 120 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  P-ERK3 (S189) polyclonal antibody detects endogenous levels of ERK3 protein only when phosphorylated at Ser189.

• Applications Notes:

  Western blot analysis of extracts of 293 cells, using Phospho-ERK3-S189 antibody.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4