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Histone H3 (Acetyl-K9) polyclonal antibody

CAT: 0384-BS8009-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS8009-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27 and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Histone H3.1; H3/a; H3/b; H3/c; H3/d; H3/f; H3/h; H3/i; H3/j; H3/k; H3/l
Swiss Prot
P68431/Q71DI3/P84243
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide including acetyl-lysine 9 of Histone H3.
Conjugation
Unconjugated
Related Pathways
Histone Lysine Methylation Pathway, Examples of Crosstalk Between Post-translational Modifications, DNA Methylation
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Acetylated
Molecular Weight
~ 17 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Ac-Histone H3 (K9) polyclonal antibody detects endogenous levels of Histone H3 protein only when acetylated at K9.
Applications Notes
Western blot (WB) analysis of Histone H3 (Acetyl-K9) pAb at 1:1000 dilution Lane1:3T3-L1 whole cell lysate (30ug) Lane2:C6 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (30ug) Lane4:A549 whole cell lysate (40ug) Lane5:LOVO whole cell lysate (40ug)
Host or Source
Rabbit

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