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DNMT3A polyclonal antibody

CAT: 0384-BS60742-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS60742-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
DNA (cytosine-5) -methyltransferase 3A; Dnmt3a; DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; M.HsaIIIA; DNMT3A
Swiss Prot
Q9Y6K1
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in Human DNMT3A
Conjugation
Unconjugated
Related Pathways
DNA Methylation
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 130 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
DNMT3A polyclonal antibody detects endogenous levels of DNMT3A protein.
Applications Notes
Western blot (WB) analysis of DNMT3A pAb at 1:500 dilution Lane1:MEF whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug) Lane4:Jurkat whole cell lysate (40ug) Lane5:PC3 whole cell lysate (40ug) Lane6:HEK293T whole cell lysate (40ug)
Host or Source
Rabbit

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