PHAX polyclonal antibody

• Background :

  Phosphorylated adapter RNA export protein, a phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC) -bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner. Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA) . Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG) -capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.

• CAS Number :

  9007-83-4

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

• Product Name Alternative :

  Phosphorylated adapter RNA export protein; RNA U small nuclear RNA export adapter protein; PHAX; RNUXA

• Swiss Prot :

  Q9H814

• Reactivity :

  Human, Rat, Mouse

• Immunogen :

  Synthetic peptide, corresponding to Human PHAX.

• Conjugation :

  Unconjugated

• Applications :

  WB ICC

• Dilution :

  WB:1:500~1:1000 ICC:1:50~1:200

• Purity :

  The protein was purified from E.coli and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 55 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -25°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  PHAX polyclonal antibody detects endogenous levels of PHAX protein.

• Applications Notes :

  Western blot (WB) analysis of PHAX polyclonal antibody at 1:500 dilution LaneA:L02 whole cell lysate LaneB:MCF-7 whole cell lysate LaneC:SP2/0 whole cell lysate LaneD:H9C2 whole cell lysate

• Host or Source :

  Rabbit