PDLIM1 polyclonal antibody

CAT: 0384-BS62275-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS62275-01Size:50 µL
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Background
CLP36, also known as PDLIM1, CLIM1 or Elfin, is a 329 amino acid cytoplasmic protein that associates with Actin stress fibers at the cytoskeleton. Expressed at high levels in skeletal muscle and heart and at lower levels in colon, small intestine, spleen, lung, placenta, kidney, liver, thymus and pancreas, CLP36 functions as a cytoskeletal protein that is thought to act as an adaptor, bringing target proteins to the cytoskeleton. Specifically, CLP36 interacts with Clik1 (a kinase) and recruits Clik1 to α-actinin-1, thereby facilitating the association of Clik1 with Actin stress fibers. CLP36 contains one PDZ domain and one LIM zinc-binding domain through which it conveys its protein-protein binding capabilities. Human CLP36 shares 88% sequence similarity with its rat counterpart, suggesting a conserved function between species.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
PDZ and LIM domain protein 1; C-terminal LIM domain protein 1; Elfin; LIM domain protein CLP-36; PDLIM1; CLIM1; CLP36
Swiss Prot
O00151
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to Human PDLIM1.
Conjugation
Unconjugated
Applications
WB IHC IP IF
Dilution
WB:1:500~1:1000 IHC:1:50~1:200 IP:1:50~1:200 IF:1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 36 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PDLIM1 polyclonal antibody detects endogenous levels of PDLIM1 protein.
Applications Notes
Western blot (WB) analysis of PDLIM1 polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:Hela whole cell lysate LaneC:H1688 whole cell lysate LaneD:The Heart tissue lysate of Mouse LaneE:The Muscle tissue lysate of Mouse LaneF:The Heart tissue lysate of Rat LaneG:The Muscle tissue lysate of Rat
Host or Source
Rabbit

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