Cyclin E1 polyclonal antibody

• Background:

  Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  G1/S-specific cyclin-E1; CCNE1; CCNE

• Swiss Prot:

  P24864

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to Human Cyclin E1.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC IF

• Dilution:

  WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 48 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Cyclin E1 polyclonal antibody detects endogenous levels of Cyclin E1 protein.

• Applications Notes:

  Western blot (WB) analysis of Cyclin E1 polyclonal antibody at 1:500 dilution LaneA:HepG2 whole cell lysate LaneB:MCF-7 whole cell lysate LaneC:K562 whole cell lysate LaneD:The Lung tissue lysate of Mouse

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4