ETS1 (Phospho-S282) polyclonal antibody

• Background:

  Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1–3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression and share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5'-GGAA-3' element. Evidence indicates that the DNA binding activity by Ets-1 is regulated at the level of phosphorylation.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Protein C-ets-1; p54; ETS1; EWSR2

• Swiss Prot:

  P14921

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic phosphopeptide derived from human ETS1 around the phosphorylation site of Serine 282.

• Conjugation:

  Unconjugated

• Related Pathways:

  Signaling Pathways Activating p38 MAP Kinase

• Applications:

  WB

• Dilution:

  WB:1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylation

• Molecular Weight:

  ~ 54 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  ETS1 (Phospho-S282) polyclonal antibody detects endogenous levels of ETS1 protein only when phosphorylated at Ser282.

• Applications Notes:

  Western blot (WB) analysis of ETS1 (Phospho-S282) polyclonal antibody at 1:500 dilution LaneA:A549 whole cell lysate LaneB:H1792 whole cell lysate

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4