CD45 (Phospho-Y1216) polyclonal antibody

• Background:

  The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing. The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates. CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling. Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; PTPRC; CD45

• Swiss Prot:

  P08575

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic phosphopeptide derived from human CD45 around the phosphorylation site of Tyrosine 1216.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB:1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylation

• Molecular Weight:

  ~ 180 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD45 (Phospho-Y1216) polyclonal antibody detects endogenous levels of CD45 protein only when phosphorylated at Tyr1216.

• Applications Notes:

  Western blot (WB) analysis of CD45 (Phospho-Y1216) polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:MCF-7 whole cell lysate LaneC:PC12 whole cell lysate LaneD:AML-12 whole cell lysate

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4