GRP78 polyclonal antibody

• Background:

  The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70, which are found in both the cytosol and nucleus of mammalian cells, play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca (2+) -binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP; HSPA5; GRP78

• Swiss Prot:

  P11021

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant full length Human GRP7.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC IF

• Dilution:

  WB: 1:500~1:2000 IHC: 1:50~1:200 IF: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 78 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  GRP78 polyclonal antibody detects endogenous levels of GRP78 protein.

• Applications Notes:

  Western blot analysis of extracts of various cell lines, using BiP/GRP78 antibody at 1:1000 dilution.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4