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MRPL21 polyclonal antibody

CAT: 0384-BS65391-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS65391-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Mitochondrial ribosomal protein L21 (MRPL21) Homo sapiens Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Multiple transcript variants encoding different isoforms were identified through sequence analysis although some may be subject to nonsense-mediated decay (NMD) . [provided by RefSeq, Jul 2008]
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Product Name Alternative
MRPL21; 39S ribosomal protein L21; mitochondrial; L21mt; MRP-L21
Swiss Prot
Q7Z2W9
Reactivity
Human, Mouse
Immunogen
The antiserum was produced against synthesized peptide derived from human MRPL21. AA range:131-180
Conjugation
Unconjugated
Applications
WB, IHC-p, IF (paraffin section), ELISA
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Modification
Unmodification
Molecular Weight
~ 23 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
MRP-L21 Polyclonal Antibody detects endogenous levels of MRP-L21 protein.
Applications Notes
Western Blot analysis of various cells using MRP-L21 Polyclonal Antibody diluted at 1: 2000
Host or Source
Rabbit

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