LYAR polyclonal antibody

• Background:

  LYAR, also named as ZLYAR, is 379 amino acid protein, which contains 2 C2H2-type zinc fingers. LYAR is expressed at at high levels in immature spermatocytes, early embryos and in fetal liver and thymus , with low expression in kidney and spleen. It is suggested that LYAR participates in regulating the stability of C23, a protein that is critical for maintaining the self-renewal and differentiation of embryonic stem cells.

• Structure Composition:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Product Name Alternative:

  LYAR; PNAS-5; Cell growth-regulating nucleolar protein

• Swiss Prot:

  Q9NX58

• Reactivity:

  Human

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human LYAR. AA range:241-290

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC-p, IF (paraffin section), ELISA

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 44 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  LYAR Polyclonal Antibody detects endogenous levels of LYAR protein.

• Applications Notes:

  Western Blot analysis of various cells using LYAR Polyclonal Antibody diluted at 1: 2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA) .

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4