SHC2 polyclonal antibody

• Background:

  Domain:The PID domain mediates binding to the TrkA receptor., function:Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons., miscellaneous:PubMed:15057824 has shown that SHC2 is poorly phosphorylated by the Trk receptors, in opposite to PubMed:12006576., PTM:Phosphorylated on tyrosines by the Trk receptors., similarity:Contains 1 PID domain., similarity:Contains 1 SH2 domain., subunit:Interacts with the Trk receptors in a phosphotyrosine-dependent manner and MEGF12. Once activated, binds to GRB2., tissue specificity:Expressed in brain. Expressed at high level in the hypothalamus and at low level in the caudate nucleus.

• Structure Composition:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Product Name Alternative:

  SHC2; SCK; SHCB; SHC-transforming protein 2; Protein Sck; SHC-transforming protein B; Src homology 2 domain-containing-transforming protein C2; SH2 domain protein C2

• Swiss Prot:

  P98077

• Reactivity:

  Human, Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human SHC2. AA range:261-310

• Conjugation:

  Unconjugated

• Applications:

  WB, ELISA

• Dilution:

  Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 59 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Sck Polyclonal Antibody detects endogenous levels of Sck protein.

• Applications Notes:

  Western blot analysis of lysates from Jurkat cells, using SHC2 Antibody. The lane on the right is blocked with the synthesized peptide.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4