CA12 polyclonal antibody

• Background:

  Carbonic anhydrase 12 (CA12) Homo sapiens Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014]

• Structure Composition:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Product Name Alternative:

  CA12; Carbonic anhydrase 12; Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; Tumor antigen HOM-RCC-3.1.3

• Swiss Prot:

  O43570

• Reactivity:

  Human

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human CA12. AA range:231-280

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC-p, IF (paraffin section), ELISA

• Dilution:

  IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 39 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For

• Specificity:

  CA XII Polyclonal Antibody detects endogenous levels of CA XII protein.

• Applications Notes:

  Western Blot analysis of NIH-3T3 293T cells using CA XII Polyclonal Antibody diluted at 1: 2000

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4