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CA12 polyclonal antibody

CAT: 0384-BS65248-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS65248-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Carbonic anhydrase 12 (CA12) Homo sapiens Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014]
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Product Name Alternative
CA12; Carbonic anhydrase 12; Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; Tumor antigen HOM-RCC-3.1.3
Swiss Prot
O43570
Reactivity
Human
Immunogen
The antiserum was produced against synthesized peptide derived from human CA12. AA range:231-280
Conjugation
Unconjugated
Applications
WB, IHC-p, IF (paraffin section), ELISA
Dilution
IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Modification
Unmodification
Molecular Weight
~ 39 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For
Specificity
CA XII Polyclonal Antibody detects endogenous levels of CA XII protein.
Applications Notes
Western Blot analysis of NIH-3T3 293T cells using CA XII Polyclonal Antibody diluted at 1: 2000
Host or Source
Rabbit

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