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GLI-3 polyclonal antibody

CAT: 0384-BS65224-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS65224-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
GLI family zinc finger 3 (GLI3) Homo sapiens This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008]
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Product Name Alternative
GLI3; Transcriptional activator GLI3; GLI3 form of 190 kDa; GLI3-190; GLI3 full length protein; GLI3FL
Swiss Prot
P10071
Reactivity
Human
Immunogen
The antiserum was produced against synthesized peptide derived from human GLI-3. AA range:11-60
Conjugation
Unconjugated
Related Pathways
Hedgehog Signaling
Applications
IHC-p, IF/ICC, ELISA
Dilution
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Modification
Unmodification
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For
Specificity
GLI-3 Polyclonal Antibody detects endogenous levels of GLI-3 protein.
Applications Notes
Immunofluorescence analysis of HepG2 cells, using GLI-3 Antibody. The picture on the right is blocked with the synthesized peptide.
Host or Source
Rabbit

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