NMDAR1 (Phospho-Ser890) polyclonal antibody

• Background:

  Glutamate ionotropic receptor NMDA type subunit 1 (GRIN1) Homo sapiens The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]

• Structure Composition:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Product Name Alternative:

  GRIN1; NMDAR1; Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NMD-R1

• Swiss Prot:

  Q05586

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human NMDAR1 around the phosphorylation site of Ser890. AA range:856-905

• Conjugation:

  Unconjugated

• Applications:

  IHC-p, IF/ICC, ELISA

• Dilution:

  Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Modification:

  Phosphorylation

• Molecular Weight:

  /

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Phospho-NMDAζ1 (S890) Polyclonal Antibody detects endogenous levels of NMDAζ1 protein only when phosphorylated at S890.

• Applications Notes:

  Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4° overnight) . High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4