Piwil2 Polyclonal Antibody

• Background:

  HILI is a 973 amino acid protein encoded by the human gene PIWIL2. HILI belongs to the argonaute family and contains one PAZ domain and one PIWI domain. HILI is a cytoplasmic protein that is expressed in adult testis and in most tumors. It regulates spermatogenesis and primordial germ cell production and has an essential role in meiotic differentiation of spermatocytes and in self-renewal of spermatogonial stem cells. Expression of HILI can modulate expression of genes involved in stem cell proliferation (such as PDGFR-b), in energy metabolism (such as Glut1), in cell-cell interaction (such as Integrin a6, GJA7, THY-1 and CD9), and in germ cell differentiation (such as STRA8) . It may also play a role as a regulatory factor of Stat3/Bcl-xS/L/CCND1 pathway. Repression of HILI can inhibit tumor cell growth. HILI acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors.

• Structure Composition:

  0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Product Name Alternative:

  HILI; Hiwi like; MILI; Miwi like; Piwi like 2; Piwi-like 2; Piwil 2; Piwil-2; Piwil2; Cancer/testis antigen 80; CT80; PIWL2_HUMAN; HILI.

• Swiss Prot:

  Q8TC59

• Reactivity:

  Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Guinea Pig

• Immunogen:

  KLH conjugated synthetic peptide derived from human Piwil2/Mili:885-973/973

• Conjugation:

  Unconjugated

• Applications:

  WB, FCM

• Dilution:

  WB=1:500-2000

• Purity:

  Affinity purified by Protein A

• Modification:

  Unmodification

• Molecular Weight:

  ~110kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -43°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Piwil2 Polyclonal Antibody detects endogenous levels of Piwil2 protein.

• Applications Notes:

  Primary: Anti- Piwil2 at 1/300 dilution

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4