KLK9Polyclonal Antibody

• Background :

  Kallikrein 9, also known as Kallikrein-Like 3 (KLK-L3), is a chymotrypsin-like serine proteinase. Kallikrein 9 was discovered as the locus for kallikreins on chromosome 19 was more fully mapped and found by similarity to the other tissue kallikreins. Kallikrein 9 has been found in the ovary, thymus, testis, prostate, skin, breast and neuronal tissues and is made by many cell lines in culture. Kallikrein 9 levels in breast cancer and uterine cancer patients have been reported to drop as the disease progresses, thus hK9 might be considered a favorable prognostic marker. Different splice variants of hK9 have been reported, although it is not yet known if they produce functional proteins. The full length Kallikrein 9 encodes for a 250 amino acid protein, with a predicted mass of 27.5 kDa and a pI of 7.53. The 234 amino acid form predicts a protein of 26 kDa with a pI of 9.76 and this quite basic pI might give the shorter form a very different function or localization. The shorter sequence also diverges before the catalytic serine residue, making it unlikely to be proteolytically active. Pre-pro-kallikrein 9 has the 17 amino acid signal sequence is removed before secretion, and the Pro-kallikrein 9 is activated to Kallikrein 9 by removal of the 5 amino acid propeptide domain.

• Structure Composition :

  0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Product Name Alternative :

  Kallikrein 8; Kallikrein 9; Kallikrein L3; Kallikrein Like 3; Kallikrein like protein 3; kallikrein related peptidase 9; Kallikrein-9; Kallikrein-like protein 3; Kallikrein8; Kallikrein9; KLK 8; KLK 9; KLK L3; KLK-L3; KLK8; KLK9; KLK9_HUMAN; KLKL 3; KLKL3.

• Swiss Prot :

  Q9UKQ9

• Reactivity :

  Human, Mouse, Rat, Horse

• Immunogen :

  KLH conjugated synthetic peptide derived from human KLK9:51-150/250

• Conjugation :

  Unconjugated

• Applications :

  IHC-P

• Dilution :

  IHC-P=1:100-500

• Purity :

  Affinity purified by Protein A

• Modification :

  Unmodification

• Molecular Weight :

  ~26kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -23°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  KLK9 Polyclonal Antibody detects endogenous levels of KLK9 protein.

• Applications Notes :

  Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK9) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4