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ZFPL1 Polyclonal Antibody

CAT: 0384-BS65830-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS65830-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Zinc finger protein-like 1 is a 310 amino acid single-pass membrane protein with two zinc fingers at the N-terminus, the second of which is likely a RING domain. The RING domain, which is a 40-60 amino acid, cysteine-rich domain that binds two atoms of zinc, plays a key role in the ubiquitination pathway. The presence of zinc finger-like and leucine zipper-like domains in ZFPL1 suggests a role in DNA binding and transcriptional regulation. ZFP1 is widely expressed in the golgi apparatus and is involved in maintaining golgi structure and regulating the rate of cargo transport.
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
D11S750; MCG4; MGC 4; ZFPL 1; Zinc finger protein like 1; Zinc finger protein MCG4; zinc-finger protein in MEN1 region
Swiss Prot
O95159
Reactivity
Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep
Immunogen
KLH conjugated synthetic peptide derived from human ZFPL1:31-130/310
Conjugation
Unconjugated
Applications
IHC-P
Dilution
IHC-P=1:100-500
Purity
Affinity purified by Protein A
Modification
Unmodification
Molecular Weight
~34kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -3°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ZFPL1 Polyclonal Antibody detects endogenous levels of ZFPL1 protein.
Applications Notes
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZFPL1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C
Host or Source
Rabbit

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