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NSF Polyclonal Antibody

CAT: 0384-BS65677-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS65677-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2) . Following membrane fusion, the α-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the α-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4) . The ATPase activity of NSF induces a conformational change in the α-SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4) .
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
N ethylmaleimide sensitive factor; N ethylmaleimide sensitive factor; N ethylmaleimide sensitive factor like protein; N ethylmaleimide sensitive fusion protein; N ethylmaleimide sensitive fusion protein; N-ethylmaleimide-sensitive fusion protein; NEM sensitive fusion protein; NEM sensitive fusion protein; NEM-sensitive fusion protein; NSF; NSF_HUMAN; SKD 2; SKD2; SKD2; Vesicle fusing ATPase; Vesicle fusing ATPase; Vesicle-fusing ATPase; Vesicular fusion protein NSF; Vesicular fusion protein NSF; Vesicular-fusion protein NSF.
Swiss Prot
P46459
Reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Zebrafish, Sheep
Immunogen
KLH conjugated synthetic peptide derived from human NSF
Conjugation
Unconjugated
Applications
WB
Dilution
WB=1:500-2000
Purity
Affinity purified by Protein A
Modification
Unmodification
Molecular Weight
83 kD
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
NSF Polyclonal Antibody detects endogenous levels of NSF protein.
Applications Notes
Sample: U937 (Human) Cell Lysate at 30 ug Spleen (Mouse) Lysate at 40 ug Lymph node (Mouse) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Primary: Anti-NSF at 1/1000 dilution
Host or Source
Rabbit

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