Products for Research Use Only

NNF1R Polyclonal Antibody

CAT: 0384-BS65456-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS65456-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
NNF1R, also called PMF1, is part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. It is required for chromosome congression and for correct operation of the spindle checkpoint. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
PMF-1; Pmf1; PMF1_HUMAN; NNF1R; Polyamine modulated factor 1; Polyamine-modulated factor 1.
Swiss Prot
Q6P1K2
Reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep
Immunogen
KLH conjugated synthetic peptide derived from human NNF1R/PMF1:101-200/300
Conjugation
Unconjugated
Applications
IHC-P
Dilution
IHC-P=1:100-500
Purity
Affinity purified by Protein A
Modification
Unmodification
Molecular Weight
34 kD
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
NNF1R Polyclonal Antibody detects endogenous levels of NNF1R protein.
Applications Notes
Paraformaldehyde-fixed, paraffin embedded (rat heart) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NNF1R) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.
Host or Source
Rabbit