STAT2 polyclonal antibody

• Background:

  Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  STAT2, Homo sapiens interferon alpha induced transcriptional activator, interferon alpha induced transcriptional activator, ISGF 3, ISGF3, MGC59816, P113, signal transducer and activator of transcription 2 113kD, Signal transducer and activator of transcription 2, STAT113, Stat2, STAT2

• Swiss Prot:

  P52630

• Reactivity:

  Human

• Immunogen:

  Synthetic peptide, corresponding to Human STAT2.

• Conjugation:

  Unconjugated

• Related Pathways:

  Jak Stat : IL-6 Receptor Signaling

• Applications:

  WB IP

• Dilution:

  WB:1:1000~1:2000 IP:1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 113 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  STAT2 polyclonal antibody detects endogenous levels of STAT2 protein.

• Applications Notes:

  Western blot (WB) analysis of Stat5a/b pAb at 1:1000 dilution Lane1:HepG2 whole cell lysate (20ug) Lane2:U-87MG whole cell lysate (20ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4