HSP70/HSPA1 polyclonal antibody

• Background:

  This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4

• Product Name Alternative:

  HSPA1A; HEL-S-103; HSP70-1; HSP70-1A; HSP70.1; HSP70I; HSP72; HSPA1; HSP70

• Swiss Prot:

  P0DMV8

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70/HSPA1 (NP_005336.3) .

• Conjugation:

  Unconjugated

• Related Pathways:

  Inhibition of Apoptosis

• Applications:

  WB IHC IF

• Dilution:

  WB 1:1000 - 1:3000 IHC 1:50 - 1:200 IF 1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 70 KDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  HSP70/HSPA1 polyclonal antibody detects endogenous levels of HSP70/HSPA1 protein.

• Applications Notes:

  Western blot analysis of extracts of various cell lines, using HSP70/HSPA1 antibody at 1:3000 dilution.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4