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DDDDK-tag polyclonal antibody

CAT: 0384-AP0007-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-AP0007-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag, ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; DDDDK
Reactivity
Transfected
Immunogen
Synthetic peptide DDDDK-tag
Conjugation
Unconjugated
Applications
WB IP IF
Dilution
WB: 1:2000~1:5000 IP: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
DDDDK-tag polyclonal antibody detects over-expressed or recombinant proteins containing the DDDDK epitope tag.
Applications Notes
Western blot (WB) analysis of DDDDK-tag pAb at 1:5000 dillution Lane1:HEK293T whole cell lysate Lane2:HEK293T whole cell lysate, transfected with pcDNA3.1-DDDDK-p62-HA.
Host or Source
Rabbit

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