Histone H3 (Phospho-T3) polyclonal antibody

• Background:

  Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; HistoneH3/l; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3C10; H3C11; H3C12; H3FJ; HIST1H3J

• Swiss Prot:

  P68431

• Reactivity:

  Human

• Immunogen:

  Synthetic phosphopeptide derived from human Histone H3 around the phosphorylation site of Threonine 3.

• Conjugation:

  Unconjugated

• Related Pathways:

  MAPK Erk in Growth and Differentiation Pathway (, Histone Lysine Methylation Pathway, NF-kB Signaling, Signaling Pathways Activating p38 MAP Kinase

• Applications:

  WB

• Dilution:

  WB: 1:1000~1:2000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylation

• Molecular Weight:

  ~ 20 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Histone H3 (Phospho-T3) polyclonal antibody detects endogenous levels of Histone H3 protein only when phosphorylated at Thr3.

• Applications Notes:

  Western blot (WB) analysis of Histone H3 (Phospho-T3) polyclonal antibody at 1:1000 dilution Lane1:MCF-7 treated with TNF-α (20 ng/ml) and Calycalin A (100nM) for 10 minutes whole cell lysate (10ug) Lane2:MCF-7 whole cell lysate (10ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4