CD16 monoclonal antibody

• Background:

  CD64 (FcgammaRI), CD32 (FcgammaRII), and CD16 (FcgammaRIII) are three classes of the immunoglobulin superfamily. CD64 has a high affinity for IgG with three Ig-like domains while CD32 and CD16 have low affinities with two Ig-like domains. Two genes encode CD16-A and CD16-B resulting only in a 6 amino acid difference in their ectodomains. However, CD16-A has a transmembrane anchor versus CD16-B, which has a glycosylphosphatidylinositol. CD64, CD32, and CD16 are membrane glycoproteins that are expressed by all immunologically active cells and trigger various immune functions (activate B cells, phagocytosis, antibody-dependent cellular cytotoxicity, immune complex clearance, and enhancement of antigen presentation) . CD16 cross-linking induces tyrosine phosphorylation (Tyr394) of Lck in NK cells. CD32 has tyrosine-based activation motifs in the cytoplasmic domain in contrast to CD16, which associates with molecules possessing these motifs.

• Structure Composition:

  Mouse IgG1. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

• Product Name Alternative:

  CD16B; FCG3; FCGR3; IGFR3; Low affinity immunoglobulin gamma Fc region receptor III-B; Fc-gamma RIII-beta; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb; FcR-10; IgG Fc receptor III-1; CD antigen CD16b

• Swiss Prot:

  O75015

• Reactivity:

  Human

• Immunogen:

  Native purified human CD16.

• Conjugation:

  Unconjugated

• Applications:

  IF, FC

• Dilution:

  IF (1/50 - 1/200)

• Purity:

  The monoclonal antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 26 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes human CD16

• Applications Notes:

  Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD16 Antibody, followed by anti-mouse IgG PE.

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4