CD294 polyclonal antibody

• Background:

  Receptor for prostaglandin D2 (PGD2) . Coupled to the G (i) -protein. Receptor activation may result in pertussis toxin-sensitive decreases in cAMP levels and Ca2+mobilization. PI3K signaling is also implicated in mediating PTGDR2 effects. PGD2 induced receptor internalization. CRTH2 internalization can be regulated by diverse kinases such as, PKC, PKA, GRK2, GPRK5/GRK5 and GRK6. Receptor activation is responsible, at least in part, in immune regulation and allergic/inflammation responses.

• Structure Composition:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  CRTH2; DL1R; GPR44; Prostaglandin D2 receptor 2; Chemoattractant receptor-homologous molecule expressed on Th2 cells; G-protein coupled receptor 44; CD294

• Swiss Prot:

  Q9Y5Y4

• Reactivity:

  Human, Rat

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD294. The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC

• Dilution:

  WB (1/500 - 1/1000), IHC (1/50 - 1/200)

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 135% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 43 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of CD294 protein.

• Applications Notes:

  Western blot analysis of CD294 expression in HEK293T (A), HuT78 (B), rat spleen (C) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4