YAP1 (phospho-S127) polyclonal antibody

• Background:

  Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637, PubMed:30447097) .

• Structure Composition:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  YAP65; Yorkie homolog; 65 kDa Yes-associated protein; YAP65

• Swiss Prot:

  P46937

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human YAP1 (pS127) . The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Related Pathways:

  Hippo Signaling

• Applications:

  WB

• Dilution:

  WB (1/500 - 1/1000)

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylation

• Molecular Weight:

  ~ 73 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of YAP1 (pS127) protein.

• Applications Notes:

  Western blot analysis of YAP1 (pS127) expression in H1688 (A) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4