IRS-4 Polyclonal Antibody

• Background:

  Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis. Interaction with GRB2 enhances insulin-stimulated mitogen-activated protein kinase activity. May be involved in nonreceptor tyrosine kinase signaling in myoblasts. Plays a pivotal role in the proliferation/differentiation of hepatoblastoma cell through EPHB2 activation upon IGF1 stimulation. May play a role in the signal transduction in response to insulin and to a lesser extent in response to IL4 and GH on mitogenesis. Plays a role in growth, reproduction and glucose homeostasis. May acts as negative regulators of the IGFI signaling pathway by suppressing the function of IRS1 and IRS2.

• Structure Composition:

  0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Product Name Alternative:

  IRS4; 160 kDa phosphotyrosine protein; Insulin receptor substrate 4; IRS 4; PY160; IRS4_MOUSE; IRS-4; Phosphoprotein of 160 kDa; pp160

• Swiss Prot:

  O14654

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  KLH conjugated synthetic peptide derived from human IRS-4: 551-650/1257

• Conjugation:

  Unconjugated

• Applications:

  WB ELISA IHC IF

• Dilution:

  WB: 1:500-1:2000 ELISA: 1:5000-1:10000 IHC: 1:100-1:500 IF: 1:100-1:500

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 134 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IRS-4 Polyclonal Antibody detects endogenous levels of IRS-4 protein.

• Applications Notes:

  Primary: Anti-IRS-4 at 1/1000 dilution.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4