SHARP1 monoclonal antibody

• Background:

  Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop) . Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity. Inhibits HNF1A-mediated transactivation of CYP1A2, CYP2E1 AND CYP3A11 (By similarity) .

• Structure Composition:

  Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  BHLHB3; DEC2; SHARP1; Class E basic helix-loop-helix protein 41; bHLHe41; Class B basic helix-loop-helix protein 3; bHLHb3; Differentially expressed in chondrocytes protein 2; hDEC2; Enhancer-of-split and hairy-related protein 1; SHARP-1

• Swiss Prot:

  Q9C0J9

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein of human SHARP1. The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB (1/1000 - 1/2000)

• Purity:

  This antibody is purified through a protein G column.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 40 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of SHARP1 protein.

• Applications Notes:

  Western blot analysis of SHARP1 expression in HeLa (A), RD (B), SHSY5Y (C), HepG2 (D), mouse brain (E), mouse skeletal muscle (F), rat skeletal muscle (G), rat brain (H), CHO (I) whole cell lysates.

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4