CGAS monoclonal antibody

• Background:

  Cyclic GMP-AMP synthase (cGAS, MB21D1) is an antiviral enzyme that produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA. The cGAS protein acts as a cytosolic DNA sensor that binds DNA and produces the cGAMP second messenger from ATP and GTP. cGAMP binds to and activates STING, a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA. STING is ubiquitously expressed and found predominantly in the ER. Following activation, STING translocates with TBK1 to perinuclear endosomes. The TBK1 kinase phosphorylates and activates interferon regulatory factors (IRFs) and NF-κB, which leads to the induction of type I interferon and other immune response genes.

• Structure Composition:

  Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  C6orf150; Cyclic GMP-AMP synthase; cGAMP synthase; cGAS; h-cGAS; Mab-21 domain-containing protein 1

• Swiss Prot:

  Q8N884

• Reactivity:

  Human

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human cGAS. The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB (1/500 - 1/1000)

• Purity:

  This antibody is purified through a protein G column.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 64 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of cGAS protein.

• Applications Notes:

  Western blot analysis of cGAS expression in Hela (A) whole cell lysates.

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4