Calreticulin monoclonal antibody

• Background:

  Calcium is a universal signaling molecule involved in many cellular functions such as cell motility, metabolism, protein modification, protein folding, and apoptosis. Calcium is stored in the endoplasmic reticulum (ER), where it is buffered by calcium binding chaperones such as calnexin and calreticulin, and is released via the IP3 Receptor channel . Calreticulin also functions as an ER chaperone that ensures proper folding and quality control of newly synthesized glycoproteins. As such, calreticulin presumably does not alter protein folding but regulates proper timing for efficient folding and subunit assembly. Furthermore, calreticulin retains proteins in non-native conformation within the ER and targets them for degradation .

• Structure Composition:

  Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  CRTC; Calreticulin; CRP55; Calregulin; Endoplasmic reticulum resident protein 60; ERp60; HACBP; grp60

• Swiss Prot:

  P27797

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein of human Calreticulin. The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB (1/500 - 1/1000)

• Purity:

  This antibody is purified through a protein G column.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 60 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of Calreticulin protein.

• Applications Notes:

  Western blot analysis of Calreticulin expression in HepG2 (A), Jurkat (B), HeLa (C), MCF7 (D), SHSY5Y (E), K562 (F), NIH3T3 (G), C6 (H), CHO (I) whole cell lysates.

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4